2020  /  2019  /  2018  /  2017  /  2016  /  2015  /  2014  /  2013  /  2012  /  2011  /  2010  /  2009  /  2008  /  pre-2008



Tien C, Yu B, Huang M, Stepien K, Sugita K, Xie X, Han L, Monnier P, Zhen M, Rizo J, Gao S, Sugita S. Open syntaxin overcomes synaptic transmission defects in diverse C. elegans exocytosis mutants. Accepted, forthcoming in Nature Communications. bioRxiv 2011-01-11. (PDF)

Susoy, V, Hung, W, Witvliet, D, Whitener, JE, Wu, M, Graham, BJ, Zhen, M, Venkatachalam, V, Samuel, ADT.
Natural sensory context drives divers brain-wide activity during C. elegans mating. bioRxiv 2020.09.10 (PDF)


Witvliet, D, Mulcahy, B, Mitchell, JM, Meirovitch, Y, Berger, DR, Wu, Y, Liu, Y, Koh, WR, Parvathala, R, Holmyard, D, Schalek, RL, Shavit, N, Chisholm, AD, Lichtman, JW, Samuel, ADT, and Zhen, M. Connectomes across development reveal principles of brain maturation in C. elegans bioRxiv 2020.04.30.066209. (PDF)


Wang, Z, Zhang, H, Zhu, L, Li, G, Li, Y, Yang, Y, Ding, Y, Zhen, M, Gao, S, Hsiai, T, Fei, P. Network-based instantaneous recording and video-rate reconstruction of 4D biological dynamics. In revision, Nature Methods; currently at bioRxiv 2020.04.17.066209 (PDF)


Yuan, W, Zhang, X, Qi, X, Hung, W, Florman, J, Huo, J, Xu, T, Xie, Y, Alkema, M., Zhen, M., Wen, Q. (2020). Flexible motor sequence generation during stereotyped escape responses. eLife 2020;9:e56942. DOI: 10.7554/eLife.56942 (PDF)


Zhao, F, Yang, Y, Li, Y, Jiang, H, Xie, X, Yu, T, Wang, X, Liu, Q, Zhang, H, Jia, H, Liu, S, Zhen, M, Zhu, D, Gao, S, Fei, P. Efficient and cost‐effective 3D cellular imaging by sub‐voxel‐resolving light‐sheet add‐on microscopy. J. Biophotonics: 13:e201960243. PMID: 32077244. DOI: (PDF)


Luyben, TT, Rai, J, Li, H, Georgiou, J, Avila, A, Zhen, M, Collingridge, GL, Tominaga, T, Okamoto, K. (2020) Optogenetic manipulation of postsynaptic cAMP using a novel transgenic mouse line enables synaptic plasticity and enhanced depolarization in the hippocampal dentate gyrus. Frontiers in Neural Circuits 14. DOI: (PDF)


Markert SM, Skoruppa M, Yu B, Mulcahy, B, Zhen, M, Gao S, Sendtner M, Stigloher C. Overexpression of an ALS-associated FUS mutation in C. elegans disrupts NMJ morphology and leads to defective neuromuscular transmission (Development; under review)

Back to top


Cuentas-Condori A, Mulcahy B, He S, Palumbos S, Zhen M, Miller DM. C. elegans neurons have functional dendritic spines. eLife. 2019 8. pii: e47918. doi: 10.7554/eLife.47918. (PDF)

Bakooshli MA, Lippmann ES, Mulcahy B, Tung K, Pegoraro E, Ahn H, Ginsberg H, Zhen M, Ashton R, Gilbert PM. A 3D Model of Human Skeletal Muscle Innervated with Stem Cell-derived Motor Neurons Enables Epsilon-subunit Targeted Myasthenic Syndrome Studies. eLife 2019;8:e44530. (PDF)


Ao Y, Zeng K, Yu B, Miao Y, Hung W, Zhen M, Yang X, Zhang Y, Gao S. An Upconversion Nanoparticle Enables Near Infrared-Optogenetic Manipulation of the C. elegans Motor Circuit. ACS Nano 13: 3373-3386. (PDF)


Huang YC, Pirri JK, Rayes D, Gao S, Mulcahy B, Grant J, Saheki Y, Francis MM, Zhen M, Alkema AJ. Gain-of-function mutations in the UNC-2/CaV2α channel lead to excitation-dominant synaptic transmission in Caenorhabditis elegans. eLife 2019;8:e45905 (PDF)

Ji N , Venkatachalam, V, Rodgers, H, Hung, W, Kawano, T, Clark, C, Lim, M., Alkema, MJ, Zhen, M, Samuel ADT. Corollary discharge promotes a sustained motor state in a neural circuit for navigation. BioRxiv 861559. (PDF)

Varga BV, Faiz M, Gao S, Linderoth E, Zhen M, Nagy A. Cortical neuroepithelial stem cell lines from human pluripotent cells. (Nature Communications; under review)

Lui Z, Wang W, Zhao X, Zhen M, Huang X. N-endrule regulation of ATGL in lipid storage. (Journal of Clinical Investigation; under review)

Back to top


Wen Q, Gao S, Zhen M. C. elegans Excitatory Ventral Cord Motor Neurons Derive Rhythm for Body Undulation. Phil. Trans. R. Soc. B. 373:20170370. (PDF)

Chitturi J, Hung W, Abdel Rahman AM, Wu M, Lim MA, Calarco JA, Baran R, Huang X, Dennis J, Zhen M. The UBR-1 Ubiquitin Ligase Regulates Glutamate Metabolism to Generate Coordinated Motor Pattern in C. elegans. PLoS Genet 14(4): e1007303. (PDF)

Gao S, Guan S, Fouad AD, Meng J, Huang Y, Li Y, Alcaire S, Hung W, Kawano T, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M. Excitatory Motor Neurons are Local Oscillators for Reverse Locomotion. Elife. 2018;7:e29915. Published 2018 Jan 23. doi:10.7554/eLife.29915 (PDF)

Xu T, Huo J, Shao S, Po M, Kawano T, Lu Y, Wu M, Zhen M, Wen Q. A descending pathway through electrical coupling facilitates undulatory wave propagation in C. elegansPNAS 115 (19): E4493-E4502. doi: 10.1073/pnas.1717022115. (PDF)

Mulcahy B, Witvliet D, Holmyard D, Mitchell J, Chisholm A, Samuel ADT, Zhen M. Volume Electron Microscopy of the Caenorhabditis elegans Nervous System. Frontiers in Neural Circuits 12:94. doi: 10.3389/fncir.2018.00094 (PDF)

Kaltdorf KV, Theiss M, Markert SM, Zhen M, Dandekar T, Stigloher C, Kollmannsberger P. Automated Classification of Synaptic Vesicles in Electron Tomographs of C. elegans using Machine Learning. PLoS One Computational Biology 13(10):e0205348. doi: 10.1371/journal.pone.0205348 (PDF

Back to top


Meng J, Ma X, Tao H, Jin X, Witvliet D, Mitchell JK, Zhu M, Dong M, Zhen M, Jin Y, Qi YB. Myrf ER-Bound Transcription Factors Drive C. elegans Synaptic Plasticity via Cleavage-Dependent Nuclear Translocation. Developmental Cell 41: 180-194. (PDF)

Opperman KJ, Mulcahy B, Giles AC, Risley MG, Bimbaum RL, Tulgren ED, Dawson-Scully K, Zhen M, Grill B. The HECT family Ubiquitin Ligase EEL-1 Regulates Neuronal Function and Development. Cell Reports 19: 822-835 (PDF)

Park S, Bin NR, Yu B, Wong R, Sitarska E, Sugita K, MA K, Xu J, Tien CW, Algouneh A, Turlova E, Wang S, Siriya P, Shahid W, Kalia L, Feng ZP, Monnier P, Sun HS, Zhen M, Gao S, Rizo J, Sugita S. UNC-18 and Tomosyn antagonistically control synaptic vesicle priming downstream of UNC-13 in C. elegans. J Neurosci 37(36): 8797-8815. (PDF)

Back to top


Ardeshiri R, Mulcahy B, Zhen M, Rezai P. A hybrid microfluidic device for on-demand orientation and multidirectional imaging of C. elegans organs and neurons. Biomicrofluidics 10: 064111 (PDF)

Lim MA, Chitturi J, Laskova V, Meng J, Findeis D, Wiekenbert A, Mulcahy B, Luo L, Li Y, Lu Y, Hung W, Qu Y, Ho C, Holmyard D, , Ji N, McWhirter R, Samuel ADT, Miller DM, Schnabel R, Calarco JA, Zhen M. Neuroendocrine modulation sustains the C. elegans forward motor state. eLife 5: e19887 (PDF)

Venkatachalam V, Ji N, Wang X, Clark C, Mitchell JK, Klein M, Tabone CJ, Florman J, Ji H, Greenwood J, Chisholm AD, Srinivasan J, Alkema MJ, Zhen M, Samuel ADT. Pan-neuronal imaging in roaming Caenorhabditis elegansPNAS 113: E1082-8 (PDF)

Park S, Bin N, Rajah MM, Kim B, Chou T, Kang SA, Sugita K, Parsaud L, Smith M, Monnier PP, Ikura M, Zhen M, Sugita S. Conformational states of syntaxin-1 govern the necessity of N-peptide binding in exocytosis of PC12 cells and Caenorhabditis elegansMolecular Biology of the Cell 27: 669-685 (PDF)

Markert SM, Britz S, Proppert S, Lang M, Witvliet D, Mulcahy B, Sauer M, Zhen M, Bessereau JL, Stigloher C. Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome. Neurophotonics 3:041802 (PDF)

Back to top


Murakami T, Qamar S, Lin JQ, Schierle GS, Rees E, Miyashita A, Costa AR, Dodd RB, Chan FT, Michel CH, Kronenberg-Versteeg D, Li Y, Yang SP, Wakutani Y, Meadows W, Ferry RR, Dong L, Tartaglia GG, Favrin G, Lin WL, Dickson DW, Zhen M, Ron D, Schmitt-Ulms G, Fraser P, Shneider NA, Holt C, Vendruscolo M, Kaminski CF, St George-Hyslop P. ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function. Neuron 88: 678-690 (PDF)

Maro GS, Gao S, Olechwier AM, Hung W, Liu M, Özkan E, Zhen M and Shen K. MADD-4/Punctin and Neurexin Organize C. elegans GABAergic Postsynapses through Neuroligin. Neuron 86: 1420-1432 (PDF) (suppl

Zhen M, and Samuel ADT. C. elegans locomotion: small circuits, complex functions. Current Opinion in Neurobiology 33: 117-126 (PDF)

Aoyagi K, Rossignol E, Hamdan FF, Mulcahy B, Xie L, Nagamatsu S, Rouleau GA, Zhen M, Michaud JL. A gain-of-function mutation in NALCN in a case with intellectual disability, ataxia, and congenital arthrogryposis. Human Mutation 36: 753-757 (PDF)

Lant B, Yu B, Goudreault M, Holmyard D, Knight JD, Xu P, Zhao L, Chin K, Wallace E, Zhen M, Gingras AC, Derry WB. CCM-3/STRIPAK promotes seamless tube extension through endocytic recycling. Nature Communications 6: 6449 (PDF)

Wang J, Chitturi J, Ge Q, Laskova V, Wang W, Li X, Ding M, Zhen M, Huang X. The C. elegans COE transcription factor UNC-3 activates lineage-specific apoptosis and affects neurite growth in the RID lineage. Development 142: 1447-57 (PDF)

Gao S, Xie L, Kawano T, Po MD, Pirri JK, Guan S, Alkema MJ, Zhen M. The NCA sodium leak channel is required for persistent motor circuit activity that sustains locomotion. Nature Communications 6: 6323 (PDF) (suppl)

Back to top


Norris AD, Gao S, Norris ML, Ray D, Ramani AK, Fraser AG, Morris Q, Hughes TR, Zhen M, Calarco JA. A pair of RNA-binding proteins controls networks of splicing events contributing to specialization of neural cell types. Molecular Cell 54: 946-59 (PDF)

Hung W, Wang Y, Chitturi J, Zhen M. C. elegans developmental decision requires insulin signaling mediated neuron-intestine communication. Development 141: 1767-79 (PDF)


Back to top


Hung W, Hwang C, Gao S, Liao E, Chitturi J, Wang Y, Li H, Stigloher C, Bessereau JL, Zhen M. Attenuation of insulin signaling contributes to FSN-1-mediated regulation of synapse development. EMBO J 32: 1745-1760 (PDF)

Qi Y, Po MD, Mac P, Kawano T, Jorgensen E, Zhen M, Jin Y. Hyperactivation of B-type motor neurons results in aberrant synchrony of the C. elegans motor circuit. Journal of Neuroscience 33: 5319-5325 (PDF)

Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Stagljar I, Nagamatsu S, Zhen M. NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion. Neuron 77: 1069-1082 (PDF)


Back to top


Wen Q, Po MD, Wyart M, Chklovskii DB, Zhen M, Samuel ADT. Proprioceptive coupling within motor neurons drives C. elegans forward locomotion. Neuron 76: 750-761 (PDF)

Najarro EH, Wong L, Zhen M, Carpio EP, Goncharov A, Garriga G, Jin Y, Ackley B. The C. elegans flamingo cadherin FMI-1 cell-nonautonomously regulates GABAergic synapse formation. Journal of Neuroscience 32: 4196-4211 (PDF)

Po MD, Calarco JA, Zhen M. Releasing the inner inhibition for axon regeneration. Neuron 73: 207-209 (PDF)

Murakami T, Yang SP, Xie L, Kawano T, Fu D, Mukai A, Bohm C, Chen F, Robertson J, Suzuki H, Tartaglia GG, Vendruscolo M, Schierle GSK, Chan FT, Moloney A, Crowther D, Kaminski CF, Zhen M, St George-Hyslop P. ALS mutations in FUS causes neuronal dysfunction and death in by a dominant gain-of-function mechanism independent of TDP-43. Human Molecular Genetics 21: 1-9 (PDF)

Back to top


Kawano TPo MDGao SLeung GRyu WSZhen M. An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Neuron 17: 572-587 (PDFSupplemental)

Mok CA, Healey MP, Shekhar T, Leroux MR, Héon E, Zhen M. Mutations in a guanylate cyclase GCY-35/GCY-36 modify Bardet-Biedl syndrome-associated phenotypes in Caenorhabditis elegans. PLoS Genetics 7: e1002335 (PDF)

Gao S and Zhen M. Action potentials drive body wall muscle contractions in Caenorhabditis elegansProceedings in the National Academy of Science USA 108: 2557-2562 (PDF)

Stigloher C, Zhan H, Zhen M, Richmond J, Bessereau JL. The presynaptic dense projection of the Caenorhabditis elegans cholinergic neuromuscular junction localizes synaptic vesicles at the active zone through SYD-2/liprin and UNC-10/RIM-dependent interactions. Journal of Neuroscience 31: 4388-4396 (PDF)

Bouhours M, Po MD, Gao S, Hung W, Li H, Georgiou J, Roder JC, Zhen M. A co-operative regulation of neuronal excitability by UNC-7 innexin and NCA/NALCN leak channel. Molecular Brain 4: 16 (PDF)

Sancar F, Touroutine D, Gao S, Oh HJ, Gendrel M, Bessereau JL, Kim H, Zhen M, Richmond JE. The dystrophin-associated protein complex maintains muscle excitability by regulating Ca(2+)-dependent K(+) (BK) channel localization. Journal of Biological Chemistry 286: 33501-10 (PDF)

Calarco JA, Zhen M, Blencowe BJ. Networking in a global world: establishing functional connections between neural splicing regulators and their target transcripts. RNA 17: 775-91 (PDF)

Ramani AK, Calarco JA, Pan Q, Mavandadi S, Wang Y, Nelson AC, Lee LJ, Morris Q, Blencowe BJ, Zhen M, Fraser AG. Genome-wide analysis of alternative splicing in Caenorhabditis elegans. Genome Research 21: 342-348 (PDF)

Souza RP, Rosa DV, Romano-Silva MA, Zhen M, Meltzer HY, Lieberman JA, Remington G, Kennedy JL, Wong AH. Lack of association of NALCN genetic variants with schizophrenia. Psychiatry Research 185: 450-452 (PDF)


Back to top


Kim JS, Hung W, Narbonne P, Roy R, Zhen M. C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and synaptic organization. Development 137: 93-102 (PDF)

Po M, Hwang C, Zhen M. PHRs: bridging axon guidance, outgrowth and synapse development. Current Opinion in Neurobiology 20: 100-107 (PDF)

Kim JSM, Hung W, Zhen M. The long and the short of SAD-1 kinase. Communicative and Integrative Biology 3: 1-5 (PDF)

Mok C, Heon E, Zhen M. Ciliary dysfunction and obesity. Clinical Genetics 77: 18-27 (PDF)


Back to top


Yeh E, Kawano T, Ng S, Fetter R, Hung W, Wang Y, Zhen M. Caenorhabditis elegans innexins regulate active zone differentiation. Journal of Neuroscience 29: 5207-17 (PDF)

Calarco JA, Superina S, O'Hanlon D, Gabut M, Raj B, Pan Q, Skalska U, Clarke L, Gelinas D, van der Kooy D, Zhen M, Ciruna B, Blencowe BJ. Regulation of vertebrate nervous system alternative splicing and development by an SR-related protein. Cell 138: 1-13 (PDF)

Back to top


Yeh E, Ng S, Zhang M, Bouhours M, Wang Y, Wang M, Hung W, Aoyagi K, Melnik-Martinez K, Li M, Liu F, Schafer WR, Zhen M. A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. PLoS Biology 6: e55 (PDF)

Liewald JF1, Brauner M, Stephens GJ, Bouhours M, Schultheis C, Zhen M, Gottschalk A. Optogenetic analysis of synaptic function. Nature Methods 5: 895-902 (PDF)

Kim JS, Lilley BN, Zhang C, Shokat KM, Sanes JR, Zhen M. A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation. Neural Development 3: 23 (PDF)

Li C, Inglis PN, Leitch CC, Efimenko E, Zaghloul NA, Mok CA, Davis EE, Bialas NJ, Healey MP, Héon E, Zhen M, Swoboda P, Katsanis N, Leroux MR. An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes. PLoS Genetics 4: e1000044 (PDF)

Gao MX, Liao EH, Yu B, Wang Y, Zhen M, Derry WB. The SCF FSN-1 ubiquitin ligase controls germline apoptosis through CEP-1/p53 in C. elegans. Cell Death and Differentiation 15: 1054-1062 (PDF)

Wang W, Bouhours M, Gracheva EO, Liao EH, Xu K, Sengar AS, Xin X, Roder J, Boone C, Richmond JE, Zhen M, Egan SE. ITSN-1 controls vesicle recycling at the neuromuscular junction and functions in parallel with DAB-1. Traffic 9: 742–754 (PDF)

Kim JS and Zhen M. Protons as intracellular messengers. Cell 132: 21-22 (PDF)

Kim JS and Zhen M. Neuronal polarity. Encyclopedia of Neuroscience, Springer-Verlag.


Back to top


Hung W, Hwang C, Po MD, Zhen M. (2007). Neuronal polarity is regulated by a direct interaction between a scaffolding protein, Neurabin, and a presynaptic SAD-1 kinase in Caenorhabditis elegans. Development 134: 237-249 (PDF)


Patel MR, Lehrman EK, Poon VY, Crump JG, Zhen M, Bargmann CI, Shen K. (2006). Hierarchical assembly of presynaptic components in defined C. elegans synapses. Nature Neuroscience 9: 1488-1498 (PDF)

Wang Y, Gracheva EO, Richmond J, Kawano T, Couto JM, Calarco JA, Vijayaratnam V, Jin Y, Zhen M. (2006). The C2H2 zinc-finger protein SYD-9 is a putative posttranscriptional regulator for synaptic transmission. Proceedings in the National Academy of Science USA 103: 10450-10455 (PDF)

Zhen M. (2006). Presynaptic differentiation. in protein trafficking in the neuron. (A.J. Bean, Ed.) London: Elsevier Academic Press. Page 75-96.

Yeh E, Kawano T, Weimer R, Bessereau JL, Zhen M. (2005). Identification of genes involved in synaptogenesis using a fluorescent active zone marker in Caenorhabditis elegansJournal of Neuroscience 25: 3833-3841 (PDF)

Liao EH, Hung W, Abrams B, Zhen M. (2004). An SCF-like ubiquitin ligase complex that controls presynaptic differentiation. Nature 430: 345-350 (PDF)

Zhen M and Jin Y. (2004). Presynaptic terminal differentiation: transport and assembly. Current Opinion in Neurobiology 14: 280-287 (PDF)

Crump JG, Zhen M, Jin Y, Bargmann CI. (2001). The SAD-1 kinase regulates presynaptic vesicle clustering and axon termination. Neuron 29: 115-129 (PDF)

Zhen M, Huang X, Bamber B, Jin Y. (2000). Regulation of presynaptic terminal organization by C. elegans RPM-1, a putative guanine nucleotide exchanger with a RING-H2 finger domain. Neuron 26: 331-343 (PDF)

Zhen M, Jin Y. (1999). The liprin protein SYD-2 regulates the differentiation of presynaptic termini in C. elegans. Nature 401: 371-37 (PDF)

Zhen M, Schein JE, Baillie DL, Candido EP. (1996). An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans. EMBO J. 15: 3229-3237 (PDF)

Zhen M, Heinlein R, Jones D, Jentsch S, Candido EP. (1993). The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation. Molecular and Cellular Biology 13: 1371-1377 (PDF)

Back to top
416-586-4800 ext 6138